Some Intresting facts about Blood or Hematology By Hetu (MSc. Medical Biochemistry)

 

 •    Eye Piece enlarges the image produced by objective lens in the microscope.

•    Resolving power of microscope is due to Objective Lens.
•    The resolving power of an oil immersion lens is 0.2 micron.
•    Refractive index of Styrax used for oil immersion objective lens is about 1.58.
•    A micron is 1000 times smaller than a millimeter.
•    Refractive index of Cedar oil used for oil immersion objective lens is about 1.52.
•    Microscope lenses can be cleaned with xylene and acetone solution.


•    In Tallquist method of hemoglobin estimation, no instrument is used.
•    Colorimeter is used in Oxy hemoglobin method and Cyanmethemoglobin method of Hb estimation

•    In Sahli’s method of Hb estimation, blood is converted into Oxyhemoglobin solution.

•    Oxyhemoglobin is detected by Sahli’s method.
•    In Sahli’s hemoglobinometer, the least count of Hb% is 0.2 gm%.
•    In Sahli’s method of Hb estimation, Upper meniscus is used for reading Hb value in Sahli’s tube
•    In Wu’s method of Hb estimation, hemoglobin is converted into Alkali hematin.
•    Acid hematin contains colloidal system of hemin and denatured globin.


•    In Haldane Gower’s Method of Hb estimation, hemoglobin is converted into carboxyhemoglobin.
•    In Cyanmethemoglobin Method of Hb estimation, reduced and oxygenated hemoglobin is estimated.

•    One gram hemoglobin contains 0.34% iron.
•    The colored pigment present in hemoglobin is known as Heme.
•    Ferrous form of iron is present in hemoglobin molecule.
•    Molecular weight of hemoglobin is about 68000 Daltons.
•    3 mg% of hemoglobin is present in the plasma.
•    If hemoglobin is present freely in plasma, then it can lead to Increased osmotic pressure.
•    The total amount of hemoglobin in the circulating blood of an adult man is about 900 gm.

million hemoglobin molecules are present in single red blood cell.
•    A₁ Hb is formed by combination of 2 alpha chain and 2 beta chain.
•    A₂ Hb has 2 alpha chain and 2 delta chain.

•    Vitamin A, Thiamine & Riboflavin can give the fluorescent emission to the tissues.

•    The total amount of hemoglobin in the circulating RBC of an adult man is about 29 pg.

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